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Expression and N-glycan analysis of human 90K glycoprotein in Drosophila S2 cells

โœ Scribed by Kim, Kyoung Ro; Kim, Yeon Kyu; Cheong, Hogyun; Kim, Jaoon Y.H.; Cha, Hyung Joon


Book ID
123129550
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
732 KB
Volume
53
Category
Article
ISSN
0141-0229

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Schneider 2 (S2) cells from Drosophila melanogaster have been used as a plasmid-based, non-lytic expression system for foreign proteins. Here, a plasmid encoding the human erythropoietin (hEPO) gene fused with a hexahistidine (His 6 ) tag under the control of the Drosophila metallothionein (MT) prom