Expression and Immunolocalization of Calcium Transport Proteins in the Canine Duodenum, Kidney, and Pancreas

Calcium–protein interactions in the extr

Calcium–protein interactions in the extracellular environment: Calcium binding, activation, and immunolocalization of a collagenase/gelatinase activity expressed in the sea urchin embryo

✍ Janice Mayne; John J. Robinson 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 329 KB

We have purified and characterized a collagenase/gelatinase activity expressed during sea urchin embryonic development. The native molecular mass was determined to be 160 kDa, while gelatin substrate gel zymography revealed an active species of 41 kDa, suggesting that the native enzyme is a tetramer