Expression and immunogenic comparison of VP2 and VP3 from marine birnavirus
β Scribed by H-J Xu; J-G Lin; C-X Zhang; S Suzuki
- Book ID
- 108860597
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 227 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0140-7775
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## Abstract The VP4, VP2, and VP1 gene regions were evaluated for their usefulness in typing human enteroviruses. Three published RTβPCR primers sets targeting separately these three gene regions were used. Initially, from a total of 86 field isolates (36 HEVβA, 40 HEVβB, and 10 HEVβC) tested, 100%
Rabbits were immunized with a synthetic heptapeptide of the sequence Arg-Asn-Arg-Ser-Ser-Arg-Ser corresponding to the carboxy-terminal region of the SV40 viral proteins VP2 and VP3. The raised antibodies recognize the viral proteins in enzyme-linked immunosorbent (ELISA) and Western blot assay. Spec