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Expression and functional analysis of the cellular retinoic acid binding protein from silkworm pupae (Bombyx mori)

✍ Scribed by Xue-Jian Wang; Jian Chen; Zheng-Bing Lv; Zuo-Ming Nie; Dan Wang; Hong-Dan Shen; Xue-Dong Wang; Xiang-Fu Wu; Yao-Zhou Zhang

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 300 KB
Volume: 102
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.21333

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✦ Synopsis

Abstract

Cellular retinoic acid binding protein (CRABP) is a member of intracellular lipid‐binding protein (iLBP), and closely associated with retinoic acid (RA) activity. We have cloned the CRABP gene from silkworm pupae and studied the interaction between Bombyx mori CRABP (BmCRABP) and all‐trans retinoic acid (atRA). The MTT assay data indicated that when BmCRABP is overexpressed in Bm5 cells, the cells dramatically resisted to atRA‐induced growth inhibition. Conversely, the cells were sensitive to atRA treatment upon knocking down the BmCRABP expression. Subcellular localization revealed that BmCRABP is a cytoplasm protein, even when treated with atRA, the CRABP still remained in the cytoplasm. These data demonstrated that the function of BmCRABP have an effect on the physiological function of atRA. J. Cell. Biochem. 102: 970–979, 2007. © 2007 Wiley‐Liss, Inc.

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