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Expression and function of P2Y receptors on Müller cells of the postnatal rat retina

✍ Scribed by Antje Wurm; Ines Erdmann; Andreas Bringmann; Andreas Reichenbach; Thomas Pannicke

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
693 KB
Volume
57
Category
Article
ISSN
0894-1491

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✦ Synopsis

In the postnatal and mature retina, many processes are controlled by the action of nucleotides. Their effects are partly mediated via activation of metabotropic P2Y receptors. However, little is known about the developmental regulation and cellular localization of P2Y receptor subtypes. Combining immunohistochemical and neurophysiological methods, we investigated the developmental expression of P2Y receptors on M€ uller cells, the principal macroglial cells of the retina. The P2Y 1 and the P2Y 4 receptors, but no other subtypes, were unequivocally localized on M€ uller cells. P2Y 1 was expressed from postnatal day 5 (P5) on and mediated a calcium response to ATP in M€ uller cells as well as a volume regulatory signaling cascade preventing M€ uller cells from swelling under hypotonic conditions. Differentiation of M€ uller cells was accompanied by a change of the calcium response pattern; the calcium responses in M€ uller cell endfeet persisted, but ATP responsiveness of M€ uller cell somata disappeared. P2Y 4 immunoreactivity was observed in M€ uller cell endfeet and synaptic terminals of rod bipolar cells from P20 on. Activated protein kinases were detected by immunohistochemistry; p-ERK occurred in M€ uller cells and amacrine cells, whereas p-Akt was detected in bipolar cells. Our data indicate that purinergic signaling via P2Y 1 and P2Y 4 receptors might contribute to differentiation processes in the postnatal retina.

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