Expression and equilibrium denaturation of cardiac troponin I: stabilization of a folding intermediate during denaturation by urea

Denaturant-induced equilibrium unfolding

Denaturant-induced equilibrium unfolding of concanavalin A is expressed by a three-state mechanism and provides an estimate of its protein stability

✍ Anindya Chatterjee; Dipak K. Mandal 📂 Article 📅 2003 🏛 Elsevier Science 🌐 English ⚖ 295 KB

The urea and guanidine hydrochloride (GdnHCl)-induced denaturation of tetrameric concanavalin A (ConA) at pH 7.2 has been studied by using intrinsic fluorescence, 8-anilino-1-naphthalenesulfonate (ANS) binding, far-UV circular dichroism (CD), and size-exclusion chromatography. The equilibrium denatu