Expression and characterization of rat surfactant protein A synthesized in Chinese hamster ovary cells
โ Scribed by Francis X. McCormack; James H. Fisher; Akira Suwabe; David L. Smith; John M. Shannon; Dennis R. Voelker
- Book ID
- 119119986
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 1010 KB
- Volume
- 1087
- Category
- Article
- ISSN
- 0167-4781
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## Abstract An expression vector was specifically designed for use in Chinese hamster ovary (CHO) cells to enhance the level of protein production in a transient expression system. Two key components that can increase protein production transiently are the promoter used to drive recombinant gene ex
The use of a temperature switch to control the growth and productivity of temperature-sensitive (ts) mutants was investigated to extend the productive life span of recombinant Chinese hamster ovary (CHO) cells in batch culture. Bromodeoxyuridine was used at 39ยฐC to select mutagenized CHO-K1 cells, w