𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression analysis and modulation by HIV-Tat of the tyrosine phosphatase HD-PTP

✍ Scribed by Massimo Mariotti; Sara Castiglioni; Jeanette A.M. Maier

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
232 KB
Volume
98
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

The human immunodeficiency virus type 1 Tat transactivates viral proteins and also affects the expression of eukaryotic genes. Since Tat is angiogenic, we assumed that the isolation of differentially expressed genes in Tat-treated endothelial cells would yield insights into the molecular mechanisms of the angiogenic process. By RNA fingerprinting, we found that Tat upregulates the tyrosine phosphatase HD-PTP mRNA in a human endothelial cell line. At the moment, little is known about HD-PTP. We here show that HD-PTP is highly conserved through evolution from yeast to man, and is ubiquitously distributed in adult and fetal tissues. HD-PTP is expressed in human cell lines derived from different tumors, but the mRNA levels do not appear to correlate with the malignant phenotype of the cells. HD-PTP mRNA was also detected in cell lines derived from tumors that develop in BKV/Tat-transgenic mice. Interestingly, a relation exists between the amounts of secreted Tat and the levels of HD-PTP mRNA. HD-PTP encodes a 185-kDa protein which is expressed in human endothelial from the umbilical cord and in human Kaposi-spindle cells. Tat-induction of HD-PTP mRNA parallels only with a slight increase of the protein, which occurs after 24 and 48 h of incubation in the presence of Tat. These results suggest that HD-PTP amounts might be regulated both at the transcriptional and post-transcriptional levels.

📜 SIMILAR VOLUMES

Inhibition of SNAP25 expression by HIV-1
Inhibition of SNAP25 expression by HIV-1 Tat involves the activity of mir-128a
✍ Davide Eletto; Giuseppe Russo; Giovanni Passiatore; Luis Del Valle; Antonio Gior 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 498 KB

## Abstract MicroRNAs (miRs) are short endogenous RNAs that regulate gene expression by incomplete pairing with messenger RNAs. An increasing number of studies show that mammalian microRNAs play fundamental roles in various aspects of cellular function including differentiation, proliferation, and

Modulation of the Na–;H antiport by insu
Modulation of the Na–;H antiport by insulin: Interplay between protein kinase C, tyrosine kinase, and protein phosphatases
✍ S. Incerpi; P. Baldini; V. Bellucci; A. Zannetti; P. Luly 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 787 KB

The insulin modulation of Na-H antiport in rat hepatocytes was studied using the fluorescent, pH-sensitive intracellular probe, 2',7' bis (carboxyethyl)-5(6)-car- boxyfluorescein (BCECF). Our data show that insulin stimulates the Na-H antiport. The dose-response of insulin effect shows a behavior ty

Activation of Src kinase by protein–tyro
Activation of Src kinase by protein–tyrosine phosphatase–PEST in osteoclasts: Comparative analysis of the effects of bisphosphonate and protein–tyrosine phosphatase inhibitor on Src activation in vitro
✍ Meenakshi A. Chellaiah; Michael D. Schaller 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 403 KB 👁 2 views

## Abstract PTP–PEST is involved in the regulation of sealing ring formation in osteoclasts. In this article, we have shown a regulatory role for PTP–PEST on dephosphorylation of c‐Src at Y527 and phosphorylation at Y418 in the catalytic site. Activation of Src in osteoclasts by over‐expression of