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Expressing genes in different Escherichia coli compartments

✍ Scribed by Pierre Cornelis

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 168 KB
Volume: 11
Category: Article
ISSN: 0958-1669
DOI: 10.1016/s0958-1669(00)00131-2

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✦ Synopsis

Production of heterologous proteins or parts thereof in different extra-cytoplasmic compartments (in the periplasm, outer membrane or extracellularly) of Escherichia coli offers multiple applications, for example, in vaccine development, immobilised enzymes and bioremediation. Nowadays, not only surface display of short peptides, but also cell-surface anchoring or secretion of functional proteins is possible. Factors influencing folding, stability and export of extra-cytoplasmic proteins are also better understood.

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