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Expressing an antibacterial protein in bacteria for raising antibodies

✍ Scribed by Philippa J Barrell; Oi Wah Liew; Anthony J Conner


Book ID
116827734
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
165 KB
Volume
33
Category
Article
ISSN
1046-5928

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A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase (D. B. Smith and K. S. Johnson, 1988, Gene 67, 31-40). Expressed fusion proteins are purified from bacterial extracts by glutathione-agarose affinity chromat