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Exposure of mammalian cells to 60-Hz magnetic or electric fields: Analysis for DNA single-strand breaks

✍ Scribed by J. A. Reese; R. F. Jostes; M. E. Frazier

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 647 KB
Volume: 9
Category: Article
ISSN: 0197-8462
DOI: 10.1002/bem.2250090305

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✦ Synopsis

Chinese hamster ovary (CHO) cells were exposed for 1 h to 60-Hz magnetic fields (0.1 or 2 mT), electric fields (1 or 38 V/m), or to combined magnetic and electric fields (2 mT and 38 V/m, respectively). Following exposure, the cells were lysed, and the DNA was analyzed for the presence of single-strand breaks (SSB), using the alkaline elution technique. No significant differences in numbers of DNA SSB were detected between exposed and sham-exposed cells. A positive control exposed to X-irradiation sustained SSB with a dose-related frequency. Cells exposed to nitrogen mustard (a known cross-linking agent) and X-irradiation demonstrated that the assay could detect cross-linked DNA under our conditions of electric and magnetic field exposures.

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