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Exploring the active site cavity of human pancreatic lipase

✍ Scribed by Damien Yann Colin; Paule Deprez-Beauclair; Maya Allouche; Robert Brasseur; Brigitte Kerfelec


Book ID
116296584
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
223 KB
Volume
370
Category
Article
ISSN
0006-291X

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## Abstract __Michael‐type additions of various thiols and Ξ±,β‐unsaturated carbonyl compounds were performed in organic solvent catalyzed by wild‐type and a rationally redesigned mutant of__ Candida antarctica __lipase B. The mutant lacks the nucleophilic serine 105 in the active‐site; this results