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Exploration of sequence space for protein engineering

✍ Scribed by Claes Gustafsson; Sridhar Govindarajan; Robin Emig

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
118 KB
Volume
14
Category
Article
ISSN
0952-3499

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✦ Synopsis

Abstract

The process of protein engineering is currently evolving towards a heuristic understanding of the sequence–function relationship. Improved DNA sequencing capacity, efficient protein function characterization and improved quality of data points in conjunction with well‐established statistical tools from other industries are changing the protein engineering field. Algorithms capturing the heuristic sequence–function relationships will have a drastic impact on the field of protein engineering. In this review, several alternative approaches to quantitatively assess sequence space are discussed and the relatively few examples of wet‐lab validation of statistical sequence–function characterization/correlation are described. Copyright © 2001 John Wiley & Sons, Ltd.

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