Explanations accounting for transduction by bacteriophage λ in maltose negative bacteriophage λ resistant mutants of Escherichia coli K-12
✍ Scribed by Braun-Breton, C. ;Hofnung, M.
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 718 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Entry of DNA from lambda phages particles into lambdarMAl- mutants of Escherichia coli K-12 is shown to be due to two distinguishable processes. One, residual transduction, results from a low level expression of lamB. The other one, background transduction, is independent of gene lamB. Interpretations are presented for these results. It is propos that residual transduction is due to a weak promoter pB3 located within or near the distal part of the gene preceeding lamB in the same operon. It is proposed that background transduction is due to a secondary receptor structure for phage lambda. Finally a tentative hypothesis relatin pB3 to insertion sequences is presented.