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Experiments in membrane protein crystallization

✍ Scribed by P. Gros; H. Groendijk; J. Drenth; W.G.J. Hol


Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
798 KB
Volume
90
Category
Article
ISSN
0022-0248

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The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bod