Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to
Experiments, correlation and prediction of protein partition coefficient in aqueous two-phase systems containing PEG and K2HPO4KH2PO4
✍ Scribed by Qinhua Peng; Zongcheng Li; Yigui Li
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 651 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0378-3812
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## Abstract Correlations to describe the effect of surface hydrophobicity and charge of proteins with their partition coefficient in aqueous two‐phase systems were investigated. Polyethylene glycol (PEG) 4000/phosphate, sulfate, citrate, and dextran systems in the presence of low (0.6% w/w) and hig
The effect of protein concentration in partitioning in PEG/ salt aqueous two-phase systems has been investigated. PEG 4000lphosphate systems in the presence of 0% wlw and 8.8% wlw NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000lphosphate system wi