Experimentally induced cancer of the colon

✍ Scribed by Dr. Eleanor E. Deschner

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 462 KB
• Volume: 34
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(197409)34:3+<824::aid-cncr2820340707>3.0.co;2-2

✦ Synopsis

1-2 Dimethylhydrazine (DMH) injected weekly a t a dose of 20 mg/kg body weight induced colon cancer in CF, mice with a n incidence of over 90% by 186 days' treatment. An unusual specificity was shown for the production of neoplasms i n the distal colon. A tritiated thymidine-labeling index curve, developed over nine treatment periods, demonstrated a marked elevation a t 87 days. At this time, in addition t o increased numbers of labeled epithelial cells, an expanded proliferative compartment closer to the luminal surface was noted. Focal atypias confined to one or two crypts were seen as early as 38 days. T h e frequency and size of these lesions within each animal increased with treatment time. Labeled indices of the focal atypias were often two to three times greater than that of the normal surrounding mucosa. A variable response among similarly treated animals indicated that multiple factors, i.e. physiological, genetic, a n d unknown, play a role i n the induction of colon cancer.

Cancer 34~824-828, 1974.