Experimental study on induction of renal cell carcinoma apoptosis by antisense oligonucleotide targeting survivin

A proteomic study reveals unspecific apo

A proteomic study reveals unspecific apoptosis induction and reduction of glycolytic enzymes by the phosphorothioate antisense oligonucleotide oblimersen in human melanoma cells

✍ Martina Stessl; Martina Marchetti-Deschmann; Johannes Winkler; Bodo Lachmann; Gü 📂 Article 📅 2009 🏛 Elsevier 🌐 English ⚖ 775 KB

The question of specificity and the elucidation of the exact molecular mechanism of action of post-transcriptional gene silencing agents are two major challenges for their establishment as therapeutics. A proteomic off-target effect study (2-DE with MS) in combination with DIGE comparing the phospho