Experimental studies on bone induction using low-molecular-weight poly (DL-lactide-co-glycolide) as a carrier for recombinant human bone morphogenetic protein-2
✍ Scribed by Bessho, K. ;Carnes, D. L. ;Cavin, R. ;Ong, J. L.
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 398 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Abstract
An appropriate carrier acting as a slow delivery vehicle for the BMPs is required for maximal clinical effectiveness of these bone‐inductive proteins. The purpose of this study was to evaluate a low‐molecular‐weight PLGA copolymer as a synthetic, biodegradable carrier for rhBMP‐2 implantation in vivo. Two, 10, or 50 μg of recombinant human BMP‐2 were mixed with 10 mg of a poly (DL‐lactide‐co‐glycolide) (PLGA) 50:50 copolymer and implanted into the calf muscles of Wistar rats. Soft X‐ray analysis and histologic examination indicated that new bone formation occurred at all rhBMP‐2‐implanted sites within 3 weeks after implantation. Correlation of rhBMP‐2 concentration with the amount of bone induction was confirmed by specific alkaline phosphatase activity and calcium content assay. In vitro analysis indicated that 78.5% of the PLGA copolymer was degraded to smaller molecular weight material after 14 days in PBS solution. It is suggested that rhBMP‐2 was released in an active form at the implant site during the degradation of the copolymer, resulting in the induction of new bone formation. Thus this low‐molecular‐weight PLGA copolymer material represents a promising delivery vehicle for BMPs, and possibly other growth factors, around dental and orthopedic implants. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 61: 61–65, 2002