✍ Scribed by Papagudi Sriram; Nicolas Kalogerakis; Leo A. Behie
No coin nor oath required. For personal study only.
data on the autolysis of trypsin at 37°C in the range 0.2 to 10 mg/l are presented. The data can be well represented by a second order kinetic rate in accordance with reaction mechanisms proposed in the literature. These data are useful in assessing the effect of loss of proteolytic activity in hybridoma cultures and establishing the dynamics of the proteolytic degradation of antibodies. Nomenclature ka -Second order reaction rate constant for autolysis reaction, l/mgd M -Concentration of trypsin at time t, mg/l Mo -Concentration of trypsin at time t=O, mg/l n -Order of reaction t -Time,d [Trl, -Enzymatically active trypsin molecule [Tr]i -Enzymatically inactive trypsin molecule Z -Concentration of the autolysis product, mg/l
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