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Experimental determination and thermodynamic modeling of phase equilibrium and protein partitioning in aqueous two-phase systems containing biodegradable salts

✍ Scribed by Brenda Perez; Luciana Pellegrini Malpiedi; Gisela Tubío; Bibiana Nerli; Pedro de Alcântara Pessôa Filho


Book ID
119292582
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
560 KB
Volume
56
Category
Article
ISSN
0021-9614

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Aqueous two-phase systems containing ure
✍ Christian Rämsch; Lutz B. Kleinelanghorst; Esther A. Knieps; Jörg Thömmes; Maria 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 200 KB 👁 2 views

During recombinant E. coli fermentation with high-expression levels inclusion bodies are often formed. Aqueous two-phase systems have been successfully used in the presence of urea for the initial recovery step of inclusion bodies from E. coli. Basic studies of the complex interactions are lacking.