๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Experimental confirmation of non-scanning fluorescence confocal microscopy using speckle illumination

โœ Scribed by Shi-hong Jiang; John G. Walker

Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
591 KB
Volume
238
Category
Article
ISSN
0030-4018

No coin nor oath required. For personal study only.

โœฆ Synopsis

A novel non-scanning fluorescence confocal microscope arrangement that uses speckle illumination is described. The arrangement gives a full-field image without the need for a scanning system but requires averaging over a large number of independent frames. A method to reduce the number of frames to give an image of acceptable quality is investigated and computer simulation results are presented that demonstrate the effectiveness of this method. Experimental results are presented that show the confocal properties of depth discrimination and improved spatial resolution.

๐Ÿ“œ SIMILAR VOLUMES

Use of a new fluorescent probe, seminaph
Use of a new fluorescent probe, seminaphthofluorescein-calcein, for determination of intracellular pH by simultaneous dual-emission imaging laser scanning confocal microscopy
โœ You Zhou; Elizabeth M. Marcus; Richard P. Haugland; Michal Opas ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 785 KB

A new p H indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular p H (pH,) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter s