Experimental cancer chemotherapy using A liver metastatic model of human colon cancer transplanted into the spleen of severe combined immunodeficient mice

✍ Scribed by Tsong-Hong Kuo; Tetsuro Kubota; Hideki Nishibori; Masahiko Watanabe; Toshiharu Furukawa; Suguru Kase; Hirokazu Tanino; Yoshiro Saikawa; Tatsuo Teramoto; Masaki Kitajima

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 499 KB
• Volume: 52
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930520207

✦ Synopsis

We have developed a liver metastatic model of human colon cancer using severe combined immunodeficient (SCID) mice. Liver metastases were observed in all the SCID mice on day 28 after intrasplenic injection with 5 X lo6 dissociated tumor cells of COL-2-JCK, a human colon cancer strain serially transplanted in nude mice. When this model was applied for chemotherapeutic experiments, 5-fluorouracil(5-FU) demonstrated significant antitumor effects in preventing liver metastases, whereas the efficacy of 5-FU was limited in the currently used sc-ip chemosensitivity assay in nude mice. When the human LDH-5 isozyme was evaluated in the homogenized metastatic liver tissue of SCID mice, a good correlation was obtained between the liver tumor weights and LDH-5 isozyme, suggesting that it could be a promising quantitative indicator for metastases. This model would be useful for further studies on the treatment of liver metastases of colon cancer.