Experimental aspects of capillary isoelectric focusing with electroosmotic zone displacement
β Scribed by Sarah Molteni; Wolfgang Thormann
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 692 KB
- Volume
- 638
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
On-line capillary zone electrophoresis/electrospray ionization mass spectrometry (CZE-ESMS) and capillary isoelectric focusing/electrospray ionization mass spectrometry (CIEF/ESMS) were employed for protein analysis. The separation mechanisms and the detection limits of CZE/ESMS and CIEF/ESMS were c
## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (MβIPG), onβline digestion by trypsinβbased immobilized enzyme microreactor (trypsinβIMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not