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Expansion of substrate specificity by rational enzyme design


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
62 KB
Volume
99
Category
Article
ISSN
0006-3592

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## Abstract Of all presently available Baeyer–Villiger monooxygenases, phenylacetone monooxygenase (PAMO) is the only representative for which a structure has been determined. While it is an attractive biocatalyst because of its thermostability, it is only active with a limited number of substrates

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We have studied the relationship between amino acid sequence and substrate specificity in a DNA glycosylase family by characterizing experimentally the specificity of four new members of the family. We show that principal component analysis (PCA) of the sequence family correctly predicts the substra