Expansion of rat 6-thioguanine-resistant T-lymphocyte clones by stimulation with ionomycin and a phorbol ester

✍ Scribed by Tao Chen; Anane Aidoo; Daniel A. Casciano; Robert H. Heflich

• Book ID: 101265049
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 137 KB
• Volume: 31
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1998)31:1<97::aid-em13>3.0.co;2-k

✦ Synopsis

Previous molecular analyses of the mutations pro-along with mitogen-free conditioned medium, was duced in the rat lymphocyte hprt assay were hin-found to expand 64% of 276 mutant clones to at least dered by difficulties encountered in growing mutant 5 x 10 5 cells in nine days of culture. Some clones were lymphocytes from 6-thioguanine-resistant clones. In expanded to more than 4 1 10 6 cells. The procedure this study, we evaluated the ability of the calcium appears suitable for propagating rat lymphocyte ionophore, ionomycin, and the tumor promotor, clones for mutation analysis. Environ. Mol. Mutagen. phorbol 12-myristate 13-acetate, to stimulate clone 31:97-102, 1998 ᭧ 1998 Wiley-Liss, Inc.* expansion. A medium containing these two agents,