Expansion of hematopoietic stem/progenitor cells
β Scribed by Wu Hai-Jiang; Deng Xin-Na; Duan Hui-Jun
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 93 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0361-8609
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The need for a rapid and reliable marker for the engraftment potential of hematopoietic stem and progenitor cell (HPC) transplants has led to the development of flow cytometric assays to quantitate such cells on the basis of their expression of CD34. The variability associated with enumeration of lo
In an effort to obtain defined culture conditions for ex vivo expansion of hematopoietic stem and progenitor cells which avoid the supplementation of serum, we cultured human CD34 + hematopoietic progenitor cells in a chemically defined, serum-free medium in the presence of hematopoietic growth fact
## Abstract Stem cell transplantation represents a critical approach for the treatment of many malignant and nonβmalignant diseases. The foundation for these approaches is the ability to cryopreserve marrow cells for future use. This technique is routinely employed in all autologous settings and is