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Expansion and long-term maintenance of induced pluripotent stem cells in stirred suspension bioreactors

✍ Scribed by Mehdi Shafa; Kirsten Sjonnesen; Akihiro Yamashita; Shiying Liu; Marek Michalak; Michael S. Kallos; Derrick E. Rancourt


Book ID
112219755
Publisher
Wiley (John Wiley & Sons)
Year
2011
Tongue
English
Weight
450 KB
Volume
6
Category
Article
ISSN
1932-6254

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Inoculation and growth conditions for the large-scale expansion of mammalian neural stem cells (NSC) have been determined. We examined suspension culture bioreactors of murine NSC, and concluded that the oxygen level should be kept high (20%), and the osmolarity of the medium should be kept low (bel