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Exon skipping and reading through stop codons: two research approaches for a therapy of Duchenne muscular dystrophy. Monaco, 17–18 January, 2004

✍ Scribed by Guenter Scheuerbrandt


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
85 KB
Volume
7
Category
Article
ISSN
1099-498X

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✦ Synopsis


Exon skipping to change Duchenne into Becker dystrophy

The exon-skipping technique tries to change a Duchenne into a Becker mutation. If a deletion or a point mutation disturbs the reading frame, and thus causes Duchenne dystrophy, the reading frame can be restored by removing one or more exons from the mRNA directly before or after the mutation.

Exons can be eliminated from the mRNA using antisense oligoribonucleotides. These are RNA structures consisting of about 20 nucleotides whose