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Exoenzyme kinetics with applications to the determination of nucleotide sequences

✍ Scribed by Charles R. Cantor; Igxacio Tinoco Jr.; Leonard Peller

Publisher
Wiley (John Wiley & Sons)
Year
1964
Tongue
English
Weight
598 KB
Volume
2
Category
Article
ISSN
0006-3525

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✦ Synopsis

The kinetics of the action of exoeneymes on linear polymers is developed. The results are applied to the problem of sequence determination in nucleic acids. It is found that a choice of experimental conditions different from the steady-state situation provides a liberation of successive nucleotides id better resolved time intervals. The inference of the nucleotide sequence of a hypothetical dodecamer is explored in detail. Calculations indicate the possibility of determining the base sequences of oligomers containing up t o 25 nucleotides.

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