Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
✍ Scribed by George B. Segel; Bruce R. Gordon; Marshall A. Lichtman; Marsha M. Hollander; Martin R. Klemperer
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 460 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
We have found that PHA produces an alteration in the lymphocyte membrane which allows ^86^Rb^+^ or ^42^K^+^ in prelabeled lymphocytes to exchange for cations present in washing solutions. These observations suggested that PHA might induce an increase in the exodus of intracellular potassium during incubation in physiologic media. We, therefore, examined ^86^Rb^+^ and ^42^K^+^ efflux from rat and human lymphocytes during incubation in tissue culture medium. The rate constant for efflux, K~e~, was significantly increased by PHA.^86^Rb^+^ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment.