Exercise-induced up-regulation ofMMP-1andIL-8genes in endurance horses
✍ Scribed by Katia Cappelli; Michela Felicetti; Stefano Capomaccio; Camillo Pieramati; Maurizio Silvestrelli; Andrea Verini-Supplizi
- Book ID
- 104497912
- Publisher
- BioMed Central
- Year
- 2009
- Tongue
- English
- Weight
- 372 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1472-6793
No coin nor oath required. For personal study only.
✦ Synopsis
Background:
The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.in this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (pbmcs).
Results:
Quantitative real-time pcr (qrt-pcr) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (mmp-1) and interleukin 8 (il-8), which were derived from previous genome-wide expression analysis. significant up-regulation of these two genes was found in 10 horses that had completed a race of 90-120 km in a time-course experimental design.
Conclusion:
These results suggest that mmp-1 and il-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.