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Excitation of Selected Proton Signals in NMR of Isotopically Labeled Macromolecules

✍ Scribed by Philippe Pelupessy; Elisabetta Chiarparin; Geoffrey Bodenhausen

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 65 KB
Volume: 138
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.1999.1715

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✦ Synopsis

In isotopically labeled macromolecules, it is possible to excite the signal of a selected proton by shuttling magnetization back and forth between the chosen proton and a heteronucleus such as 13 C or 15 N, using two-way doubly selective heteronuclear cross-polarization. Selective excitation of a chosen proton can be followed by homonuclear coherence transfer to identify side-chain resonances of the corresponding amino acid in proteins. The resulting onedimensional experiments yield information that can usually only be obtained from three-dimensional HSQC-TOCSY spectra. The method also provides efficient suppression of solvent signals without affecting resonances close to the solvent peak.

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