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Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida

โœ Scribed by Siegert, P.; McLeish, M. J.; Baumann, M.; Iding, H.; Kneen, M. M.; Kenyon, G. L.; Pohl, M.


Book ID
111680216
Publisher
Oxford University Press
Year
2005
Tongue
English
Weight
416 KB
Volume
18
Category
Article
ISSN
1741-0126

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## Abstract __Alteration of the substrate specificity of thiamin diphosphate (ThDP)โ€dependent benzoylformate decarboxylase (BFD) by errorโ€prone PCR is described. Two mutant enzymes, L476Q and M365Lโ€L461S, were identified that accept__ ortho__โ€substituted benzaldehyde derivatives as donor substrates

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The thiamin diphosphateand Mg 2 -dependent enzyme benzoylformate decarboxylase (BFD) from Pseudomonas putida was characterized with respect to its suitability to catalyze the formation of chiral 2-hydroxy ketones in a benzoin-condensation type reaction. Carboligation constitutes a side reaction of B