Exchange of colicin receptor capacity between strains ofEscherichia colisensitive or resistant to colicin K-K235
✍ Scribed by David P. Brunner; Gary S. Graham; Ronald W. Treick
- Book ID
- 104760358
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 789 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
Phage and colicin-resistant mutants were derived from Escherichia coIi K-12 P678. Two classes of phage T6 and colicin K-resistant mutants (genotype tsx) were isolated. Tsx-2 mutants, which demonstrated mucoid growth and increased sensitivities to many antibiotics, became sensitive to colicin K when pretreated with ethylenediaminetetraacetate (EDTA), whereas Tsx-1 mutants did not. Reassociation of EDTAreleased material partially restored resistance to colicin K for Tsx-2 mutants. When EDTA-released material from strain P678 was associated with either class of K-resistant mutant, an increase in colicin K sensitivity resulted. Observations suggest that colicin K can act on its target site once it penetrates the cell surface. In addition, results suggest that functional colicin K receptors can be transferred from sensitive to resistant strains, thus conferring colicin sensitivity.