𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis

✍ Scribed by Pavel Bouchal; Igor Kučera

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
422 KB
Volume
44
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

The well-known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for modern proteomic approaches. We describe here improved preparation conditions that allow good separation and quantitative analyses of hundreds of membrane or periplasmic proteins. To illustrate this optimized procedure, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented.

📜 SIMILAR VOLUMES

Analysis of proteins from membrane-enric
Analysis of proteins from membrane-enriched cerebellar preparations by two-dimensional gel electrophoresis and mass spectrometry
✍ Giulia Friso; Lilian Wikström 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 183 KB 👁 2 views

Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the