Ex vivo expansion of CD34+cells and immunocytes from umbilical cord blood
β Scribed by Yaming Wei; Xiumei Lin; Ping Mao; Jiongcai Lan
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 450 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1610-1979
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## Abstract Since umbilical cord blood (UCB), contains a limited hematopoietic stem/progenitor cells (HSC) number, successful expansion protocols are needed to overcome the hurdles associated with inadequate numbers of HSC collected for transplantation. UCB cultures were performed using a human str
In an effort to obtain defined culture conditions for ex vivo expansion of hematopoietic stem and progenitor cells which avoid the supplementation of serum, we cultured human CD34 + hematopoietic progenitor cells in a chemically defined, serum-free medium in the presence of hematopoietic growth fact