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Ex vivo expansion of CD34+cells and immunocytes from umbilical cord blood

✍ Scribed by Yaming Wei; Xiumei Lin; Ping Mao; Jiongcai Lan


Publisher
Springer
Year
2006
Tongue
English
Weight
450 KB
Volume
5
Category
Article
ISSN
1610-1979

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Initial CD34+ cell-enrichment of cord bl
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## Abstract Since umbilical cord blood (UCB), contains a limited hematopoietic stem/progenitor cells (HSC) number, successful expansion protocols are needed to overcome the hurdles associated with inadequate numbers of HSC collected for transplantation. UCB cultures were performed using a human str

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In an effort to obtain defined culture conditions for ex vivo expansion of hematopoietic stem and progenitor cells which avoid the supplementation of serum, we cultured human CD34 + hematopoietic progenitor cells in a chemically defined, serum-free medium in the presence of hematopoietic growth fact