## Abstract Numerous processes in cells can be traced by using fluorescence resonance energy transfer (FRET) between two fluorescent proteins. The novel FRET pair including the red fluorescent protein TagRFP and kindling fluorescent protein KFP for sensing caspaseโ3 activity is developed. The lifet
โฆ LIBER โฆ
Evolutionary optimization of fluorescent proteins for intracellular FRET
โ Scribed by Nguyen, Annalee W; Daugherty, Patrick S
- Book ID
- 109908345
- Publisher
- Nature Publishing Group
- Year
- 2005
- Tongue
- English
- Weight
- 274 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1066
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## Abstract ## Background In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-
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