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Evolution of methionine initiator and phenylalanine transfer RNAs

✍ Scribed by B. Larue; R. J. Cedergren; D. Sankoff; H. Grosjean


Book ID
105293091
Publisher
Springer
Year
1979
Tongue
English
Weight
702 KB
Volume
14
Category
Article
ISSN
0022-2844

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Alanine and phenylalanine tRNA sequences were amplified by PCR from Arabidopsis thaliana nuclear DNA using degenerate oligonucleotides which introduced specific mutations into the acceptor stem. The aminoacylation of T7 RNA polymerase transcripts of these sequences was investigated in vitro using pa