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Evolution, Mechanisms, and Applications of Intein-mediated Protein Splicing

โœ Scribed by Perler, F. B.; Allewell, N. M.


Book ID
124060664
Publisher
The American Society for Biochemistry and Molecular Biology
Year
2014
Tongue
English
Weight
578 KB
Volume
289
Category
Article
ISSN
0021-9258

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Protein splicing in trans results in the ligation of two protein or peptide segments linked to appropriate intein fragments. We have characterized the trans-splicing reaction mediated by a naturally expressed, approximately 100-residue N-terminal fragment of the Mycobacterium tuberculosis intein and