Evidence that the same structural gene encodes testicular and adrenal 3β-hydroxysteroid dehydrogenase-isomerase
✍ Scribed by John R. D. Stalvey; Miriam H. Meisler; Anita H. Payne
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 494 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0006-2928
No coin nor oath required. For personal study only.
✦ Synopsis
Thermostability of 33-hydroxysteroid dehydrogenase-isomerase (33HSD) activity was examined in testes and adrenal glands from several inbred lines and feral mice. A thermolabile varant of 33HSD was detected in the feral Brno mice. The thermostability (ti/2) of 33HSD was approximately 7 rain for both testes and adrenal glands from C57BL/6J mice, compared with 4 min for both tissues from Brno mice. Comparison of testicular and adrenal 33HSD thermostability in six kinds of mice indicated that the tl/2 of 33HSD was correlated in the two tissues and could be classified into two distinct types, thermolabile and thermostable. In contrast, quantitative variants in 33HSD activity were not correlated in the two tissues. These data are consistent with the hypothesis that testicular and adrenal 33HSD is encoded by the same structural gene but that expression of 33HSD activity is independently controlled in testes and adrenal glands.