✦ LIBER ✦
Evidence that the methylation inhibitor cycloleucine causes accumulation of a discrete ribosomal RNA precursor in hamster mitochondria
✍ Scribed by Daniel L. Prince; Robert M. Kotin; Donald T. Dubin
- Book ID
- 104766886
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 348 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0301-4851
No coin nor oath required. For personal study only.
✦ Synopsis
A novel RNA fraction, 'Cy RNA,' that accumulates in mitochondria when hamster cells are treated with the methylation inhibitor cycloleucine, has been characterized by high resolution acrylamide gel electrophoresis and DNA-RNA hybridization. Cy RNA ran in gels as a discrete band, with an apparent chain length of 2 600. It hybridized specifically to restriction fragments containing genes for the mitochondrial ribosomal RNAs. We infer that Cy RNA is a discrete polycistronic ribosomal RNA precursor transcript, whose processing is dependent on normal methylation.