Evidence that the CD45 phosphatase regulates the activity of the phospholipase C in mouse T lymphocytes

✍ Scribed by Jacqueline Marvel; Galia Rimon; Peter Tatham; Shamshad Cockcroft

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 613 KB
• Volume: 21
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830210129

✦ Synopsis

The importance of the tyrosine phosphatase CD45 in the regulation of lymphocyte activation was first demonstrated using antibodies against the extracellular domain of CD45 in functional assays. More recently it was reported that CD45-negative mutants were nonresponsive to stimulation through the Tcell receptor-CD3 complex. We have studied the effect of CD4S cross-linking on the early signals induced by CD3 in mouse Tcells. We show that CD45 cross-linking inhibits the increase in inositol phosphates and cytoplasmic Ca7+ induced by cross-linking of CD3. This indicates that CD45 is involved in the regulation of phospholipase C.