✦ LIBER ✦
Evidence that the axolemmal mitogen for cultured schwann cells is a positively charged, heparan sulfate proteoglycan-bound, heparin-displaceable molecule
✍ Scribed by Dr. M. A. Decoster; G. H. Devries
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 543 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
✦ Synopsis
Treatment of axolemma with pH 9 buffer results in a pellet enriched two-fold in the mitogen for cultured Schwann cells. Heparitinase treatment releases 8 % of the mitogen into solution, while heparin selectively solubilizes the mitogen, resulting in an extract which has a specific mitogenic activity approximately 2.5 times greater than the mitogenicity of the starting axolemma membrane. These data support a model in which the axolemmal mitogen is a positively charged molecule associated with negatively charged sulfated proteoglycans.