Evidence that blocking factors in the sera of multiparous mice are associated with immunoglobulins

✍ Scribed by J. Tamerius; I. Hellström; K. E. Hellström

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: French
• Weight: 690 KB
• Volume: 16
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910160313

✦ Synopsis

Previous work has shown that lymph-node cells from pregnant BALBIc mice can kill syngeneic tumor cells in a microcytotoxicity assay, and that sera from pregnant BALBIc mice can block (inhibit) that effect. 7he present study shows that passage of such sera through immunoadsorbents charged with anti-mouse IgG2a or IgCjb removed the blocking activity, and that this could be recovered in the eluates; passage through an anti-IgG, immunoadsorbent was less effective in decreasing blocking serum activity and adsorption to immunoadsorbent charged with anti-IgM or anti-lgA did not remove it at all. Passage of blocking serum through an immunoadsorbent coupled with antibodies from a rabbit anti-mouse embryo antiserum removed all of its blocking activity, and this could be recovered in the eluates. The findings are compatible with the hypothesis that the blocking factors operating in this system are largely complexes between embryonic antigens and IgC,, and IgC2b.