Evidence of Red Cell Alignment in the Magnetic Field of an NMR Spectrometer Based on the Diffusion Tensor of Water
✍ Scribed by Philip W Kuchel; Christopher J Durrant; Bogdan E Chapman; Penelope S Jarrett; David G Regan
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 159 KB
- Volume
- 145
- Category
- Article
- ISSN
- 1090-7807
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✦ Synopsis
The alignment of human erythrocytes in aqueous suspensions in the magnetic field B(0) (called the z-direction) of an NMR spectrometer was shown by calculating the diffusion tensor for water in the sample. The diffusion was measured using a pulsed-field-gradient spin-echo NMR method. The extent of diffusion anisotropy for water was exemplified by the values of the apparent diffusion coefficients with erythrocytes of normal shape and volume: for a typical experiment the values for the x-, y-, and z-directions were (6.88 +/- 0.17) x 10(-10), (7.07 +/- 0.17) x 10(-10), and (10.20 +/- 0.17) x 10(-10) m(2) s(-1), respectively. Cells in hypo- and hyperosmotic media were also studied and they too showed the anisotropy of the apparent diffusion coefficients but the extents were different. A new method of data analysis was developed using the Standard Add-On Packages in a Mathematica program. The experimental findings support evidence of erythrocyte alignment that was previously obtained with a high-field-gradient q-space method.