Evidence of Biofilm on Adenoid and Sinus Mucosa in Pediatric Chronic Rhinosinusitis

✍ Scribed by Elizabeth Dunham; Hassan Ramadan; Karen H Martin

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 224 KB
• Volume: 121
• Category: Article
• ISSN: 0023-852X
• DOI: 10.1002/lary.22295

✦ Synopsis

Objectives: This study attempts to analyze and quantify biofilm presence on pediatric adenoid and sinus samples from children with chronic rhinosinusitis using confocal microscopy to evaluate specimens of adenoid and sinus mucosa in 39 pediatric patients undergoing sinus surgery, adenoidectomy or both, for treatment of obstructive and/or infectious problems.

Study Design: Prospective

Methods: Mucosal specimens were harvested on the day of planned surgery, frozen, sectioned and then double stained for biofilm glycocalyx and DNA. Specimens were imaged and analyzed using confocal microscopy for the presence of biofilm on the mucosal epithelial surface. Findings were correlated to patient diagnosis and basic demographic information.