Evidence for thein vivoregulation of glucose 6-phosphate dehydrogenase activity inHydrogenomonas eutropha H 16from measurements of the intracellular concentrations of metabolic intermediates
✍ Scribed by B. Bowien; A. M. Cook; H. G. Schlegel
- Book ID
- 104759142
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 563 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
The inhibiton of fructose utilization by whole cells of Hydrogenomonas eutropha H 16, following the addition of hydrogen to the gas phase, has been explained as an inhibition of glucose 6-phosphate dehydrogenase (Blackkolb and Schlegel, 1968a, b).
The intracellular concentrations of glucose 6-phosphate, 6-phosphogluconate, three inhibitors of the enzyme (NADH, ATP and phosphoenolpyruvate) and some related metabolites were measured in cells incubated in the presence and absence of hydrogen.
Inhibiton of glucose 6-phosphate dehydrogenase was confirmed by an increase in the glucose 6-phosphate pool and a decrease in the 6-phosphogluconate concentration. The regulatory control is apparently due to a threefold increase in the NADH concentration while the concentrations of the other two inhibitors fell slightly. When the measured intracellular concentrations of intermediates were used in the in vitro assay of glucose 6-phosphate dehydrogenase activity, an almost total inhibition of the dehydrogenase was observed, therefore further regulatory factors must be considered.