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Evidence for the utilization of extracellular [γ-32P]ATP for the phosphorylation of intracellular proteins in the squid giant axon

✍ Scribed by PANT, H; TERAKAWA, S; YOSHIOKA, T; TASAKI, I; GAINER, H


Book ID
122643294
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
497 KB
Volume
582
Category
Article
ISSN
0304-4165

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A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P