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Evidence for the Rate-Limiting Departure of the 5‘-Oxygen in Nonenzymatic and Hammerhead Ribozyme-Catalyzed Reactions

✍ Scribed by Zhou, De-Min; Usman, Nassim; Wincott, Francine E.; Matulic-Adamic, Jasenka; Orita, Masaya; Zhang, Li-He; Komiyama, Makoto; Kumar, Penmetcha K. R.; Taira, Kazunari


Book ID
126436051
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
201 KB
Volume
118
Category
Article
ISSN
0002-7863

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## Abstract Recent theoretical studies of the alkaline hydrolysis of the amide bond have indicated that the nucleophilic attack of the hydroxide ion at the carbonyl carbon of the amide group is rate limiting. This is shown to be inconsistent with a large amount of experimental observations where th